(Solved): 1.You are studying the function of a 200-amino acid bacterial protein whose wild-type carboxyl termi ...

1.You are studying the function of a 200-amino acid bacterial protein whose wild-type carboxyl terminal sequence is: -G-E-N-E-T-I-C-S_COOH (In other words, the terminal “S” is the last, or 200^th amino acid of the protein.)

(a). You feed a mutagen that induces base substitutions; you recover and sequence a mutant form of the protein.  It’s wild-type in sequence—199/200 amino acids the same, but one amino-acid substitution in the carboxyl terminus: -G-E-N-E-R-I-C-S_COOH (substitution in bold, codon 197).  Show the mutant change at the level of the mRNA codon, comparing wild-type to mutant, and name the mutation type.

b. You mutagenize wild type a second time and recover a new mutant protein that’s shorter than wild-type, but the sequence that’s there is wild-type.  It’s 195 amino acids in length.  Show the mutant change at the level of the mRNA codon, comparing wild-type to mutant, and name the mutation type.

c. You mutagenize wild type again, this time with a mutagen that induces base additions or deletions during DNA replication.  You recover a mutant protein whose sequence is wild type through and including amino acid #194 (E).  The mutant protein’s carboxyl terminal sequence, starting with amino acid #193 (G) for reference, is:

-G-E-M-K-Q-Y-A-H-K_COOH and is 201 amino acids in length.  Align relevant mRNA sequences comparing wild type and mutant, showing CLEARLY the mutant change.  Name the mutation type.