(Solved): 1193pts /1900 Question 7 of 19 Stacked Part H C Macmillas Learning Suppose you can synthesize a sho ...

/1900 Question 7 of 19 Stacked Part H C Macmillas Learning Suppose you can synthesize a short duplex DNA fragment with any sequence you desire. With this synthetic fragment and the procedures described in parts A through G, you could remove an EcoRI restriction site from a DNA molecule and incorporate a new BamHI restriction site at approximately the same location. There are at least two different methods for replacing an EcoRI restriction site with a BamHI restriction site. Complete the passage describing the method that inserts a DNA fragment with blunt ends. Cut the DNA with

then treat the DNA with

to create blunt ends. Then ligate a synth

BamIII. with the

recognition sequence between the two resulting blunt ends. BeoRI, A second method would be to synthesize a DNA fragment that would ligate efficiently to the sticky ends generated by EcoRI cleavage and introduce a BamHI cleavage site, without regenerating the EcoRI cleavage site. Select the synthetic DNA fragment you could use to replace an EcoRI restriction site with a BamHI restriction site.

GATCGAATTC-3' 3'-CTTAAGCTAG-5'

TTAAGGATCC

-CCTAGGAATT-5'

-AATTGGATCC-3' 3'-CCTAGGITAA-5'

5'-AATTCCTAGG-3' 3'-GGATCCTTAA-5'