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(Solved): 3. A 10.067g sample of cereal is placed in a 250mL Erlenmeyer flask along with 1 -g of sodium as ...
3. A 10.067g sample of cereal is placed in a 250−mL Erlenmeyer flask along with 1 -g of sodium ascorbate, 40−mL of ethanol, and 10−mL of 50%w/vKOH. After refluxing for 30 minutes, 60−mL of ethanol is added, and the solution is cooled to room temperature. Vitamin A is extracted using three 100−mL portions of hexane. The combined portions of hexane are evaporated, and the residue containing vitamin A is transferred to a 5−mL volumetric flask and diluted to volume with methanol. A standard addition is prepared in a similar manner using a 10.093g sample of the cereal and spiking it with 0.0200−mg of vitamin A. Injecting the sample and standard addition into the HPLC gives peak areas of 6.77×103 and 1.32×104, respectively. Report the vitamin A content of the sample in milligrams per 100g cereal.