(Solved): A goneralized trandoction experiment is set op se that a plape is carrying a donor senit with the ge ...
A goneralized trandoction experiment is set op se that a plape is carrying a donor senit with the genofpe mert'
(b)/(0^( ))ohr
^( ). This doeor thenin is used to tramdace a rociplent
Ecoll strin with the penorype mer' hio
^(-)-trr-. After manstuction, the recipicse popelation is plated ee a minimal medium containing only organic salts and a simple carton soterce supplemented wibh methionine aed biotin. A large namber of colonies are obtained. The wild-type genes indicate the bacteria do not need a supplemented mixial molfen in onder to grow. Any bacteria with mutant genes will require a supplement of methicnine, bictin, or theoolies lo goos. The ese of minimal medium without all the requied nutrients is a type of marker selection. Marker seloction utilizes He inakility of some basteria with mutame genolypes to grow en minimal medium by creating a medium that celly select bacterin can pow one. Select the porsible genotypes of the colonies that grew on this mediun. mer tie
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