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(Solved): Analyze and answer: 1.If you see a band in the electrophoresis gel in the sample 1 lane and it is t ...
Analyze and answer:
1.If you see a band in the electrophoresis gel in the sample 1 lane and it is the same size as the band that appears in the lane where the E. coli bacteria control is placed on the gel, what does it mean?
2. On the other hand, if you don't see a band the same size as the positive control on the gel, what does it mean?
3. If you have bands in the negative control (distilled water), what could have happened?
\begin{tabular}{|l|} \hline Carril y Muestra \\ 1 Marcador de 200 bp ADN \\ 2 E. coli control positivo \\ 3 Control negativo \\ 4 Control Escherichia coli \\ 5 Control Serratia marcesens \\ 6 Control Bacillus subtilis \\ 7 Muestra de agua de rio 1 \\ 8 Muestra de agua 2 \\ 9 Muestra de agua 3 \\ \hline \end{tabular}
Expert Answer
1. If indeed the bands are the identical size as that of the positive control, template contamination is probably the cause. Starting with fresh reagents would be the easiest course of action. 2. The am