Demonstrate the affect of frame shift mutations on an amino acid sequence. 1. Transcribe and translate the “normal” DNA strand below. 2. Note that the second nucleotide of the second triplet was left out in the mutant strand. 3. Transcribe and translate the mutant DNA. Normal DNA T A C G T C T T C G A C T C T Normal mRNA Blank 1 Blank 2 Blank 3 Blank 4 Blank 5 Normal Amino Acids START Glutamine Lysine Leucine Arginine Mutant DNA T A C G C T T C G A C T Mutant mRNA A U G C G A A G C U G A Amino Acids Blank 6 Blank 7 Blank 8 Blank 9