(Solved): Dus: Sun, Oct 19 Assessment 67 pts Resources Solution Al Tutor (1) Try Again juestion 7 of 19 ...

Dus: Sun, Oct 19 Assessment 67 pts Resources Solution Al Tutor (1) Try Again 原兩 juestion 7 of 19 Stacked

Suppose you can synthesize a short duplex DNA fragment with any sequence you desire. With this synthetic fragment and the procedures described in parts

through

, you could remove an EcoRI restriction site from a DNA molecule and incorporate a new BamHI restriction site at approximately the same location. There are at least two different methods for replacing an EcoRI restriction site with a BamHI restriction site. Complete the passage describing the method that inserts a DNA fragment with blunt ends. Cut the DNA with

then treat the DNA with

to create blunt ends. Correct Answer Correct Answer Then ligate a synthetic fragment with the

recognition sequence between the two resulting blunt ends. Correct Answer A second method would be to synthesize a DNA fragment that would ligate efficiently to the sticky ends generated by EcoRI cleavage and introduce a BamHI cleavage site, without regenerating the EcoRI cleavage site. Select the synthetic DNA fragment you could use to replace an EcoRI restriction site with a BamHI restriction site. O

GATCGAATTC

-CTTAAGCTAG-5'

5'-TTAAGGATCC-3'

-CCTAGGAATT-5'

AATTGGATCC

-CCTAGGTTAA-5*

-AATTCCTAGG-3' 3'-GGATCCTTAA-5' vicomed Answer