(Solved): Effect of pH on Enzyme Activity 5.4 Effect of \( \mathrm{pH} \) on Enzyme Activity Pre-Lab 9. How do ...

5.4 Effect of \( \mathrm{pH} \) on Enzyme Activity Pre-Lab 9. How do temperature and \( \mathrm{pH} \) have similar effects on enzymes? 10. Which tube \( (1,2 \), or 3 ) do you expect to have the most enzyme activity in the Effect of \( \mathrm{pH} \) experiment? Each enzyme has a pH at which the speed of the reaction is optimum (occurs best). Any higher or lower \( \mathrm{pH} \) affects hydrogen bonding and the structure of the enzyme, leading to reduced activity or denaturing of the enzyme. Catalase is an enzyme found in cells where the \( \mathrm{pH} \) is near 7 (called neutral \( \mathrm{pH} \) ). Other enzymes prefer different \( \mathrm{pHs} \). The pancreas secretes a slightly basic ( \( \mathrm{pH} 8-8.3 \) ) juice into the digestive tract, and the stomach wall releases a very acidic digestive juice, which can be as low as \( \mathrm{pH} 2 \). Experimental Procedure: Effect of \( \mathrm{pH} \) Number three clean test tubes and use the appropriate transfer pipet to add solutions to the test tubes as follows: 1. To each tube, add \( 1 \mathrm{ml} \) of catalase. 2. To tube 1 , add \( 2 \mathrm{ml} \) of water adjusted to \( \mathrm{pH} 3 \). Swirl to mix. 3. To tube 2, add \( 2 \mathrm{ml} \) of water adjusted to \( \mathrm{pH} 7 \). Swirl to mix. 4. To tube 3 , add \( 2 \mathrm{ml} \) of water adjusted to \( \mathrm{pH} 11 \). Swirl to mix. 5. Wait 1 minute. Now add \( 4 \mathrm{ml} \) hydrogen peroxide to each tube, and swirl to mix before noting the height of the bubble column. 6. Record your results in Table 5.4, and plot your results in Figure 5.4. Figure \( 5.4 \) Effect of \( \mathrm{pH} \) on enzyme activity. Conclusion: Effect of \( \mathrm{pH} \) - What is your conclusion concerning the effect of \( \mathrm{pH} \) on enzyme activity?