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(Solved): Given the template DNA, show step - by - step to design a forward primer and reverse primer (both in ...


Given the template DNA, show step - by - step to design a forward primer and reverse primer (both in 5' direction) 

5’ ATGACGGAATATAAGCTGGTGGTGGTGG---GGCTGCATGAGCTGCAAGTGTGTGCTCTCCTAA 3’

3’ TACTGCCTTATATTCGACCACCACCACC---CCGACGTACTCGACGTTCACACACGAGAGGATT 5’



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Solution- Step 1 The Polymeras Chain Reaction or PCR has results in the selective amplification of a specific region of a DNA molecule and so also can be used to generate a DNA fragment for cloning. The basic principle underlying is when a double sta
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