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(Solved): i mostly need help with c) for the first one and the polyadenylation signal for queston 2 a and the ...
i mostly need help with c) for the first one and the polyadenylation signal for queston 2 a and the kozak's sequence
1. Use the prokaryotic gene DNA sequence below to answer the following questions: a. Write the corresponding sequences, circle \& label them in the sequence above: -35 consensus sequence: TTGACA (label as - 35) -10 consensus sequence (Pribnow box): TA TAAT (label as PB) Shine-Delgarno sequence in the corresponding mRNA: AGGAGGU (label as SD) Start (initiation) codon in the corresponding mRNA: AUG (label as start) Stop (termination) codon in the corresponding mRNA: (label as stop) b. What region of this prokaryotic DNA sequence will be transcribed into mRNA? Circle one. 1−120 35-108 54-146 (72-146) c. What will be the nascent polypeptide sequence translated from this mRNA? d. Where is the 3′ UTR? Circle one. 12−7235−72 6.9-146 121−146 II. Use the eukaryotic gene DNA sequence below to answer the following questions:
a. Write the corresponding sequences, circle \& label them in the sequence above: TATA box sequence: TATAA (label as TATA) Polyadenylation signal in the corresponding mRNA: (label as poly-A) KozaK's sequence in the corresponding mRNA: (label as Kozak) Start (initiation) codon in the corresponding mRNA: A+G (label as start) Stop (termination) codon in the corresponding mRNA: (label as stop) b. What region of mRNA contains the open reading frame that will be translated into protein? Circle one. 51-111 61.69 37.45 71-81) c. Where is the 3′ UTR? Circle one. 70-111 14. 60 73-90 85-150