In order to investigate the relationship between the electron transport chain and ATP synthesis, a researcher sets up preparations in intact mitochondria that are capable of cellular respiration if supplies with an oxidizable substrate, like succinate.
The following preparations were made and oxygen consumption and ATP synthesis were measured.
Prep 1 succinate only
Prep 2 succinate plus the complex I inhibitor rotenone
Prep 3 succinate plus the complex IV inhibitor cyanide
Prep 4 succinate plus the uncoupling molecule dinitrophenol (DNP)
Prep 5 succinate plus the ATP synthase inhibitor oligomycin
Use this information to answer each of the following questions.
For each preparation would oxygen be consumed? yes or no
prep 1
prep 2
prep 3
prep 4
prep 5
Would ATP be synthesized? yes or no
prep 1
prep 2
prep 3
prep 4
prep 5