(Solved): Primer designing 2: A single-stranded DNA sequence that codes for a protcin are shown below (shaded ...

Primer designing 2: A single-stranded DNA sequence that codes for a protcin are shown below (shaded blue). Design two PCR primers ( 18 nucleotides long each) with restriction sites, EcoRI and BamHI (see New England Biolabs for restriction sites and sequences), added at \( 5^{\prime} \) and \( 3^{\prime} \) ends, respectively, for the amplification and cloning of this gene into a plasmid cut open with the same restriction enzymes (4 points). ATGGCTAAAGGCGGAGCT CCCGGGTTCA AAGTCGCAAT ACTTGGCGCT GCCGGTGGCA TTGGCCAGCCCCTTGCGATGTTGATGAAGATGAATCCTCTGGTTTCTGTTCTACATCTATATGA TGTAGTCAATGCCCCTGGTGTCACCGCTGATATTAGCCACATGGACACGGGTGCTGTGGTGCG TGGATTCTTGGGGCAGCAGCAGCTGGAGGCTGCGCTTACTGGCATGGATCTTATTATAGTCCC TGCAGGTGTTCCTCGAAAACCAGGAATGACGAGGGATGATCTGTTCAAAATAAACGCAGGAATT GTCAAGACTCTGTGTGAAGGGATT'GCAAAGTGTTTGTCCAAGAGCCATTGTCAACCTGATCAGTA ATCCTGTGAACTCCACCGTGCCCATCGCAGCTGAAGTTTTCAAGAAGGCTGGAACTTATGATCC AAAGCGACTTCTGGGAGTTACAATGCTCGACGTAGTCAGAGCCAATACCTTTGTGGCAGAAGTA TTGGGTCTTGATCCTCGGGATGTTGATGTTCCAGTTTGTTGGCGGTCATGCTGGTGTAACCATT TTGCCCCTTCTATCTCAGGTGAAGCCTCCAAGTTCTTTCACACAAGAAGAGATTAGTTACCTGA CTGATAGGATTCAAAATGGTGGAACAGAAGTTGTCGAGGCCAAAGCAGGAGCTGGTTCAGCAAC TCTCTCAATGGCTTATGCTGCCGTTAAGTTTGCAGATGCATGCCTCAGGGGCTTAAGAGGAGAT GCTGGTGTCATTGAATGCGCGTTTGTGTCTTCTCAGGTGACTGAACTTCCATTCT1TGCATCAA AAGTACGACTTGGTCGCAATGGTATCGAAGAAGTATACTCCCTTGGCCCGCTAAATGAGTATGA GAGGATTGGATTGGAGAAAGCGAAGAAAGAGTTGGCAGGAAGCATTGAGAAGGGAGTTTCCTT CATCAGAAGCTAA