(Solved): Q1. You have isolated a plasmid from E. coli and wish to begin your analysis of it by making a restr ...

Q1. You have isolated a plasmid from E. coli and wish to begin your analysis of it by making a restriction map. Using three restriction enzymes, B (BamHI), E(EcoRI), N (NotI), you perform six different digestions: single digests using each enzyme alone and double digests using each combination of two enzymes. Agarose gel electrophoresis of the resulting fragments produces the results shown here. Draw a final restriction map of the plasmid showing your solution strategy. (10 Marks)