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(Solved): Question 6 1 pts Besides being different in chemical structure from dNTPs, what other modification w ...



Question 6 1 pts Besides being different in chemical structure from dNTPs, what other modification was made to ddNTPs to make them useful in traditional Sanger chain termination sequencing? ddNTPs were modified so that they cannot be incorporated into a DNA molecule by the polymerase. ddNTPs were modified so that they could be more frequently incorporated into the DNA molecule during the DNA sequencing reaction over that of dNTPs. ddNTPs were modified to be larger than dNTPs, so they would distort the shape of the double helix if used to make a DNA molecule. ddNTPs were labeled with a radioactive tag, so that after the sequencing reaction was complete, the resulting DNA in each of the four tubes could be separated by size using polyacrylamide gel electrophoresis and the gel then exposed to X -ray film so that the sequence could be read.



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