(Solved): Restriction enzyme mapping. An insert (thick orange line) has been cloned into the EcoRI (R) and Hi ...

Restriction enzyme mapping. An insert (thick orange line) has been cloned into the EcoRI (R) and HindIII (H) sites of a 5 Kbp vector (thin black line), with the resulting construct diagrammed below. Restriction enzyme digests were done to determine the location of Bam HI and Xbal sites within the insert (there are none in the vector). The gel with the restriction enzyme digests in shown on the right. Answer the questions below the figure. 1. (2p) What size, in kilobases, is the insert? (answer is an integer) 2. (2p) On the plasmid diagram, which letter indicates the position of the N -terminus of the b-galactosidase protein? 3. (2p) If bacteria transformed with this plasmid are plated onto medium containing \( x \)-gal and inducer (IPTG), what color will the colonies be? 4. (2p) How many Xbal sites (X) are there in the total plasmid? (answer is an integer) 5. (3p) What size bands would you expect to get with a B/X double digest? (list as decreasing sizes to nearest 0.5 kb , no units, with commas between, for example: \( 9.5,7.0,0.5) \)