(Solved): Selected (12 pts) Valuable reagents. The following reagents are often used in protein chemistry: CNB ...

Selected (12 pts) Valuable reagents. The following reagents are often used in protein chemistry: CNBr Urea Mercaptoethanol Trypsin Performic acid Dabsyl chloride 6 N HCl Ninhydrin Phenyl isothiocyanate Chymotrypsin Which one is the best suited for accomplishing each of the following tasks? (a) Determination of the amino acid sequence of a small peptide. (b) Identification of the amino-terminal residue of a peptide (of which you have less than

). (c) Reversible denaturation of a protein devoid of disulfide bonds. Which additional reagent would you need if disulfide bonds were present? (d) Hydrolysis of peptide bonds on the carboxyl side of aromatic residues. (e) Cleavage of peptide bonds on the carboxyl side of methionines. (f) Hydrolysis of peptide bonds on the carboxyl side of lysine and arginine residues. 3. Protein Isolation (9pts) Describe in a little detail three basic methods to isolate proteins from a complex mixture of proteins. A. B. C.