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(Solved): The enzyme \alpha -chymotrypsin is secreted in the pancreas of mammals and cleaves peptide bonds bet ...
The enzyme
\alpha -chymotrypsin is secreted in the pancreas of mammals and cleaves peptide bonds between certain amino acids. Several solutions containing the small peptide
N-glutaryl-l-phenylalanine-
p-nitroanilide at different concentrations were prepared and all samples contained
\alpha -chymotrypsin (
[E]_(0)) at a concentration of
1.50\times 10^(-3)mmol.dm^(-3). The following data were obtained on the initial rates of the formation of product: \table[[
(S)/(mmoldm^(-3)),0,334,0,450,0,667,1,00,1,33,1,67],[
(v)/(mmoldms^(-3)),0,150,0,199,0,285,0,406,0,516,0,619]] Calculate the Michaelis constant
(K_(M))and catalytic efficiency
(\eta ).20p