To determine the Km of the enzyme, we need to use the Michaelis-Menten equation: where V is the velocity of the reaction, [S] is the substrate concentration, Vmax is the maximum velocity of the reaction, and Km is the Michaelis constant.We can rearrange the equation to get: The concept used in this problem is Michaelis-Menten kinetics, which relates the initial reaction rate of an enzyme-catalyzed reaction to the concentration of substrate using the Michaelis constant (Km).