There are four enzymes of gluconeogenesis that circumvent the irreversible steps in ghycolysis. When starting with the substrate pyruvate or lactate they are Glycerol kinase, glycerol-3-phosphate dehydrogenase, fructose-2,6-blisphosphatase, and glucose-6-phosphatase Pyruvate carboxylase, phosphoenolpyruvate carboxykinase, fructose-1,6-bisphosphatase, and hexokinase Hexokinase, phosphofructokinase-1, phosphofructokinase-2 and pyruvate kinase Amino transferase, phosphoenolpyruvate carboxykinase, fructose-2,6-bisphosphatase, and glucose-6-phosphatase Pyruvate carboxylase, phosphoenolpyruvate carboxykinase, fructose-1,6-bisphosphatase, and glucose-6-phosphatase