You amplify a target gene responsible for the malfunction of apoptosis pathways in colon cells which results in colon cancer. This gene is 1461 bp in size. Can this gene be inserted into the pUC19 vector using the two enzymes you were assigned in experiment 6 AND be correctly expressed? Why or why not? (remember what expression requires: both transcription to RNA and translation to protein).

Restriction Enzymes: BamHI-HF and EcoRI-HF and BamHI-HF and ScaI-HF

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You amplify a target gene responsible for the malfunction of apoptosis pathways in colon cells which results in colon cancer. This gene is 1461 bp in size. Can this gene be inserted into the pUC19 vector using the two enzymes you were assigned in experiment 6 AND be correctly expressed? Why or why not? (remember what expression requires: both transcription to RNA and translation to protein).

Restriction Enzymes: BamHI-HF and EcoRI-HF and BamHI-HF and ScaI-HF

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