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(Solved): Your first experiment in biochemistry lab is to analyze a mixture with the use of gel filtration. Yo ...



Your first experiment in biochemistry lab is to analyze a mixture with the use of gel filtration. You are instructed to run a Sephadex G-50 column on the mixture containing the components listed below. All three components are colored, so you are able to observe the separation. The components are dissolved in water, and the column is eluted with water. a) Blue dextran, a blue dye (MW = about 2,000,000) b) Cytochrome c, a red protein (MW = 12,400) c) Flavin adenine dinucleotide (FAD), a yellow coenzyme (MW = 830) Predict the results of your experiment.

 



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Gel filtration using sephadex G-50 column is a method to saperate compone
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